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hy 15141 tbid r d systems  (R&D Systems)


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    Structured Review

    R&D Systems hy 15141 tbid r d systems
    Hy 15141 Tbid R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+tbid/Recombinant+Human+BID+(Caspase-8-cleaved)+Protein%2C+CF/pm39571574-726-76-78
    Average 93 stars, based on 16 article reviews
    hy 15141 tbid r d systems - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Selective targeting of apoptosis proteins by structurally-stabilized and/or cysteine-reactive NOXA peptides
    Article Snippet: .. Lysate samples (0.5 mg) were incubated with 0.25 μM recombinant tBID (R&D Systems) and 5 μM biotinylated NOXA SAHBs for 6 h at RT. .. The mixtures were then subjected to HA or FLAG immunoprecipitation, followed by western analysis using 1:1000 dilutions of HA (Sigma Aldrich, #12CA5), FLAG (Sigma-Aldrich, F7425), and BID (Santa Cruz sc-11423) antibodies.

    Article Title: Precision Targeting of BFL-1/A1 and an ATM Co-dependency in Human Cancer
    Article Snippet: .. Lysate samples (0.5 mg) were incubated with 0.25 μM recombinant tBID (R&D Systems) and 5 μM biotinylated NOXA SAHBs for 6 hr at room temperature. .. The mixtures were then subjected to HA or FLAG immunoprecipitation using 30 μL of pre-washed anti-HA agarose (Thermo Fisher Pierce) or anti-FLAG M2 affinity agarose (Sigma-Aldrich) beads, respectively, followed by western analysis using 1:1000 dilutions of HA (Sigma-Aldrich, #12CA5), FLAG (Sigma-Aldrich, #F7425), and BID (Santa Cruz, #sc-11423) antibodies.

    Article Title: ATR Plays a Direct Antiapoptotic Role at Mitochondria Which Is Regulated by Prolyl Isomerase Pin1
    Article Snippet: .. The cytochrome c assay was performed by subjecting isolated mitochondria to incubation with recombinant tBid (R&D Systems, 883-M8) according to Luo et al. (1998) . ..

    Article Title: Drp1-dependent mitochondrial fission via MiD49/51 is essential for apoptotic cristae remodeling
    Article Snippet: .. The semi-intact cells were then incubated for 60 min at 30°C with 75 nM recombinant tBid (R&D Systems) with or without 20 μM ionomycin (Wako) in buffer A to induce cytochrome c and Smac/DIABLO release. .. The semi-intact cells were fixed with 4% PFA, completely permeabilized by incubation with 0.1% Triton X-100 for 5 min, and subjected to immunofluorescence analysis with antibodies to cytochrome c or Smac/DIABLO.

    Recombinant:

    Article Title: Selective targeting of apoptosis proteins by structurally-stabilized and/or cysteine-reactive NOXA peptides
    Article Snippet: .. Lysate samples (0.5 mg) were incubated with 0.25 μM recombinant tBID (R&D Systems) and 5 μM biotinylated NOXA SAHBs for 6 h at RT. .. The mixtures were then subjected to HA or FLAG immunoprecipitation, followed by western analysis using 1:1000 dilutions of HA (Sigma Aldrich, #12CA5), FLAG (Sigma-Aldrich, F7425), and BID (Santa Cruz sc-11423) antibodies.

    Article Title: Precision Targeting of BFL-1/A1 and an ATM Co-dependency in Human Cancer
    Article Snippet: .. Lysate samples (0.5 mg) were incubated with 0.25 μM recombinant tBID (R&D Systems) and 5 μM biotinylated NOXA SAHBs for 6 hr at room temperature. .. The mixtures were then subjected to HA or FLAG immunoprecipitation using 30 μL of pre-washed anti-HA agarose (Thermo Fisher Pierce) or anti-FLAG M2 affinity agarose (Sigma-Aldrich) beads, respectively, followed by western analysis using 1:1000 dilutions of HA (Sigma-Aldrich, #12CA5), FLAG (Sigma-Aldrich, #F7425), and BID (Santa Cruz, #sc-11423) antibodies.

    Article Title: ATR Plays a Direct Antiapoptotic Role at Mitochondria Which Is Regulated by Prolyl Isomerase Pin1
    Article Snippet: .. The cytochrome c assay was performed by subjecting isolated mitochondria to incubation with recombinant tBid (R&D Systems, 883-M8) according to Luo et al. (1998) . ..

    Article Title: Bax transmembrane domain interacts with prosurvival Bcl-2 proteins in biological membranes
    Article Snippet: .. To study Smac release in isolated mitochondria in vitro, mitochondrial outer membrane permeabilization was induced with recombinant tBid (R&D Systems; 10 nM). ..

    Article Title: Drp1-dependent mitochondrial fission via MiD49/51 is essential for apoptotic cristae remodeling
    Article Snippet: .. The semi-intact cells were then incubated for 60 min at 30°C with 75 nM recombinant tBid (R&D Systems) with or without 20 μM ionomycin (Wako) in buffer A to induce cytochrome c and Smac/DIABLO release. .. The semi-intact cells were fixed with 4% PFA, completely permeabilized by incubation with 0.1% Triton X-100 for 5 min, and subjected to immunofluorescence analysis with antibodies to cytochrome c or Smac/DIABLO.

    Isolation:

    Article Title: ATR Plays a Direct Antiapoptotic Role at Mitochondria Which Is Regulated by Prolyl Isomerase Pin1
    Article Snippet: .. The cytochrome c assay was performed by subjecting isolated mitochondria to incubation with recombinant tBid (R&D Systems, 883-M8) according to Luo et al. (1998) . ..

    Article Title: Bax transmembrane domain interacts with prosurvival Bcl-2 proteins in biological membranes
    Article Snippet: .. To study Smac release in isolated mitochondria in vitro, mitochondrial outer membrane permeabilization was induced with recombinant tBid (R&D Systems; 10 nM). ..

    In Vitro:

    Article Title: Bax transmembrane domain interacts with prosurvival Bcl-2 proteins in biological membranes
    Article Snippet: .. To study Smac release in isolated mitochondria in vitro, mitochondrial outer membrane permeabilization was induced with recombinant tBid (R&D Systems; 10 nM). ..

    Membrane:

    Article Title: Bax transmembrane domain interacts with prosurvival Bcl-2 proteins in biological membranes
    Article Snippet: .. To study Smac release in isolated mitochondria in vitro, mitochondrial outer membrane permeabilization was induced with recombinant tBid (R&D Systems; 10 nM). ..



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    R&D Systems recombinant human tbid
    a, Chemical structure of BAI1. b, Microscale thermophoretic analysis of BAI1 binding BAX. ΔFnorm, normalized change in fluorescence. Representative of three independent experiments. c, Lack of an effect of BAI1 (0.1 μM) on early conformational changes in BAX activation in staurosporine (STS)-treated MEFs as assessed by exposure of 6A7 epitope. Three independent experiments each with three independent immunoprecipitations per group. One-way ANOVA, *P = 0.0141, NS, P > 0.05. d, BAI1 inhibits BAX insertion into the OMM. Only noninserted BAX can be recovered from isolated mitochondria by treatment with strong alkali (n = 4 independent experiments). One-way ANOVA, *P = 0.0301, ***P = 0.0002. e,f, Inhibition of BAX mitochondrial translocation by BAI1. e, Representative images of immunofluorescence for BAX translocation in WT MEFs. f, Quantification of percentage of cells with BAX puncta (n = 4, 11, 9, 11 and 12 images). Data are representative of five independent experiments. One-way ANOVA, *P = 0.0237, ****P < 0.0001. g, BAI1 (10 μM) inhibits BAX-dependent cytochrome c release as assessed by ELISA. Mitochondria isolated from BAX/BAK DKO MEFs were incubated with <t>recombinant</t> BAX and <t>tBID</t> (n = 3 independent mitochondrial isolates per group). Representative of three independent experiments. One-way ANOVA, **P = 0.0016, ##P = 0.0074. Data in c,d,f,g are presented as mean ± s.e.m. Unprocessed images of blots are provided as source data.
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    Image Search Results


    a, Chemical structure of BAI1. b, Microscale thermophoretic analysis of BAI1 binding BAX. ΔFnorm, normalized change in fluorescence. Representative of three independent experiments. c, Lack of an effect of BAI1 (0.1 μM) on early conformational changes in BAX activation in staurosporine (STS)-treated MEFs as assessed by exposure of 6A7 epitope. Three independent experiments each with three independent immunoprecipitations per group. One-way ANOVA, *P = 0.0141, NS, P > 0.05. d, BAI1 inhibits BAX insertion into the OMM. Only noninserted BAX can be recovered from isolated mitochondria by treatment with strong alkali (n = 4 independent experiments). One-way ANOVA, *P = 0.0301, ***P = 0.0002. e,f, Inhibition of BAX mitochondrial translocation by BAI1. e, Representative images of immunofluorescence for BAX translocation in WT MEFs. f, Quantification of percentage of cells with BAX puncta (n = 4, 11, 9, 11 and 12 images). Data are representative of five independent experiments. One-way ANOVA, *P = 0.0237, ****P < 0.0001. g, BAI1 (10 μM) inhibits BAX-dependent cytochrome c release as assessed by ELISA. Mitochondria isolated from BAX/BAK DKO MEFs were incubated with recombinant BAX and tBID (n = 3 independent mitochondrial isolates per group). Representative of three independent experiments. One-way ANOVA, **P = 0.0016, ##P = 0.0074. Data in c,d,f,g are presented as mean ± s.e.m. Unprocessed images of blots are provided as source data.

    Journal: Nature cancer

    Article Title: A small-molecule allosteric inhibitor of BAX protects against doxorubicin-induced cardiomyopathy

    doi: 10.1038/s43018-020-0039-1

    Figure Lengend Snippet: a, Chemical structure of BAI1. b, Microscale thermophoretic analysis of BAI1 binding BAX. ΔFnorm, normalized change in fluorescence. Representative of three independent experiments. c, Lack of an effect of BAI1 (0.1 μM) on early conformational changes in BAX activation in staurosporine (STS)-treated MEFs as assessed by exposure of 6A7 epitope. Three independent experiments each with three independent immunoprecipitations per group. One-way ANOVA, *P = 0.0141, NS, P > 0.05. d, BAI1 inhibits BAX insertion into the OMM. Only noninserted BAX can be recovered from isolated mitochondria by treatment with strong alkali (n = 4 independent experiments). One-way ANOVA, *P = 0.0301, ***P = 0.0002. e,f, Inhibition of BAX mitochondrial translocation by BAI1. e, Representative images of immunofluorescence for BAX translocation in WT MEFs. f, Quantification of percentage of cells with BAX puncta (n = 4, 11, 9, 11 and 12 images). Data are representative of five independent experiments. One-way ANOVA, *P = 0.0237, ****P < 0.0001. g, BAI1 (10 μM) inhibits BAX-dependent cytochrome c release as assessed by ELISA. Mitochondria isolated from BAX/BAK DKO MEFs were incubated with recombinant BAX and tBID (n = 3 independent mitochondrial isolates per group). Representative of three independent experiments. One-way ANOVA, **P = 0.0016, ##P = 0.0074. Data in c,d,f,g are presented as mean ± s.e.m. Unprocessed images of blots are provided as source data.

    Article Snippet: Isolated mitochondria (40 μg) from BAX/BAK DKO MEFs were incubated with 25 nM recombinant human BAX, 10 nM recombinant human tBID (R&D Systems, 882-b8–050) with or without 10 μM BAI1 at 37 °C.

    Techniques: Binding Assay, Fluorescence, Activation Assay, Isolation, Inhibition, Translocation Assay, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Incubation, Recombinant